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In a comparative study using thigh skin in women with aged skin, participants applied topical GHK-Cu alongside vitamin C and retinoic acid over 12 weeks, with collagen improvement measured through biopsy-based markers

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Methodologically, GHK-Cu research has used cell-culture systems (primarily fibroblasts, keratinocytes, and dermal papilla cells), animal models (rodent wound-healing models predominantly), human clinical-research studies in cosmetic contexts, and analytical biochemistry approaches