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Supernatants were assayed for total protein concentration with Bradford assay (BioRad 5000006) and 50100 g of protein was mixed with loading buffer and loaded in a 12 or 16% gradient gel (Thermo 1Fisher XP00162)

Differences among treatment means were determined using the StudentNewmanKeuls (SNK) multiple comparison test

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Culture of previously uncultured members of the human gut microbiota by culturomics

Durchsteche mit der Entnahmespritze das graue Septum und ziehe die Spritze mit der gewnschten Menge Serum auf

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