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Each component is studied for a different layer of the regenerative cycle: GHK-Cu for copper-mediated extracellular-matrix and collagen signaling, BPC-157 for angiogenic and growth-factor pathways, and TB-500 (acetylated Thymosin Beta-4) for actin-driven cell migration

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Following incubation, the 143B cells were fixed and blocked with 3% bovine serum albumin (BSA) and then treated with anti-hypoxyprobe antibody and DAPI