2.9 Cell culture and treatment Mouse colon epithelial cells (MCECs) were cultured in high-glucose Dulbeccos Modified Eagle Medium, supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (P/S), in a humidified incubator with 5% CO 2 at 37C
GSH exerts antioxidant activity, so anything which hampers this can have significant consequences
Foods that are naturally rich in Vitamin C, like orange juice, citrus fruits, green vegetables, strawberries, and tomatoes, all increase levels of glutathione in the body
Storage Store the lyophilized powder at 20C in a standard laboratory freezer, protected from light, moisture, and heat
Collagen formation, on the other hand, naturally declines with age or exposure to environmental stressors
Figure 4 Figure 5 In Figure 4 RNA expression overview with data from TCGA is given