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Briefly, islets were incubated overnight in full media containing 1 M thapsigargin 100 nM exendin-4 at 37 C and subsequently incubated in 25 M H 2 O 2 Substrate diluted in H 2 O 2 Substrate Dilution Buffer plus 1 M thapsigargin 100 nM exendin-4 in a final volume of 100 L for 3 hours at 37 C in a 5% CO 2 incubator