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doi: 10.1038/ejcn.2014.46

Administration of high-fat diet promotes the production of ROS, along with the elevation of tumor necrosis factor-alpha (TNF-) and adipokine secretion, thereby resulting in chronic inflammation [41]

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For measurement of metabolite levels in cells, hepatocytes were harvested by treating with 0.25 % trypsin (Gibco #25200), washing with ice-cold PBS to remove trypsin, suspending released hepatocytes in ice-cold PBS, and centrifuging the cell suspension at 1600 g for 10 min at 4 C to obtain the pelleted cells, which were immediately frozen and stored at 80 C for later analysis

Dairy products, grains, and eggs contribute substantially to dietary iodine intakes in the US