Primary antibody (anti-GCSc 1:10 (SantaCruz, sc390811) and anti-CD45 1:200 (Proteintech, 31243-1-AP)) incubation was carried out overnight at 4 C
doi: 10.1167/iovs.06-0026 171 Gnana-PrakasamJPVeeranan-KarmegamRCoothankandaswamyVReddySKMartinPMThangarajuMet al
Nevertheless, compared to all other Cys-GSTs, DHARs have a nine amino acid insertion before the 1 helix and thus the active site motif (Figure 3)
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Among these, the MUC5B promoter variant (rs35705950) is the most prominent, conferring a greater than threefold increased risk for IPF and exceeding the effect of all other known variants combined [21, 22]