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According to Severin et al ., serum urea concentration increased for the first 4 d after MTX administration and then decreased and returned to normal levels by the eighth day

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To obtain plasma, typically whole blood is collected into tubes pre-coated with an anticoagulantheparin and EDTA being the most commonand then transported to the laboratory on wet ice where the whole blood is centrifuged to fraction the sample into its component parts (red blood cells, white blood cells, and plasma)

E., Singh, R., Cannarella, R., Sengupta, P., & Dutta, S