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Cells were harvested in PBS after 48 hours of transfection, and pellets were resuspended with lysis buffer supplemented with protease inhibitor cocktail (Sigma), 0.1 mM PMSF and 1 mM DTT

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2,7-10 Thus, there remains an urgent need for large-scale, long-term randomized controlled trials to confirm the causal relationship between EGT supplementation and clinical endpoints, such as dementia conversion rates and cardiovascular events