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Apoptosis and cell cycle analysis To further facilitate our understanding about their cytotoxicity towards ATC cells, we also analyzed the cellular responses evoked by the AP probe-net using multiparameter flow cytometry (Fig

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Cells were then placed on ice, washed with cold HBSS, and incubated for 10 minutes in cold acetic acid + 150 mM NaCl buffer, pH 2.9, to strip surface ligand

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