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NADP reduction and glutathione oxidation, represented an opportunity to benchmark the recently introduced biosensor NERNST against NAPstars and roGFP2-Grx1 probes in vivo

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Iron depletion induces hepatic secretion of biliary lipids and glutathione in rats

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These two substitutions , the Nle4 and D-Phe7 modifications and were introduced through the systematic structure-activity relationship programme in Victor Hrubys laboratory at the University of Arizona that established the pharmacophore requirements for melanocortin receptor agonism and produced MT-1 as a more potent, metabolically stable, and pharmacologically tractable research tool than native -MSH