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Expression levels of the (A) AMPK and (B) SIRT6 genes and their associated proteins were analyzed using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting, and -actin was used as the loading control

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10.1016/j.metabol.2010.03.010 56

To assess the effect of cisplatin + RSL3 in eliminating senescent cells, we stained sections for SA--galactosidase or took advantage of SenTraGor