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Membranes were blocked with 5% skimmed milk-PBS at room temperature for 1 h and then incubated overnight at 4 C with primary antibodies (diluted 1:1000 in 5% BSA-TBST)

Part of the explanation may be where the study appeared

Potential Complications and Management of Small and Medium-sized CMN Potential Complications and Management of Small and Medium-sized CMN The risk for the development of cutaneous melanoma within small and medium-sized CMN is controversial and is thought to be 1% or less over a lifetime