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It has been reported that GFP fluorescence can respond to subtle environmental differences caused by POI unfolding (before aggregation), and the resulted changes in intensity (decrease in those cases) can be used directly to monitor unfolding by differential scanning fluorimetry 80

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88,100,140,141 However, some studies have also found that CHD8 is a positive regulator of the Wnt/-catenin signalling pathway in NPCs and negatively regulates this pathway in nonneuronal cell lines, suggesting that CHD8 may regulate Wnt signalling in a cell-specific manner