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Cells were harvested by centrifugation, resuspended in phosphate-buffered saline (PBS) buffer (140 mM NaCl, 2.7 mM KCl, 10 mM NaHPO, 1.8 mM KHPO, pH 7.4, and 10% glycerol), and lysed by sonication

If tests show deficiency, find out if its dietary or due to malabsorption

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Ieva E, Trapani A, Cioffi N et al (2009) Analytical characterization of chitosan nanoparticles for peptide drug delivery applications

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It plays a role in many functions, including antioxidant defenses, nutrient metabolism, the regulation of cellular events (including gene expression and cell proliferation), and immune response