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Methods EV-F3 production and purification BHK-21 cells were grown to 80% confluency before infection with EV-F3 virus stocks in Glasgow modified Eagles medium (G-MEM BHK-21, Gibco), supplemented with 2% fetal horse serum (Thermo Fisher Scientific) and minimal essential medium non-essential amino acids (MEM NEAA, Gibco)

BIOBANQUES BB-0033-00097) for retrieving samples

It should not be used as a substitute for a medication, compounded drug, or clinical-trial product

Confocal microscopy (CLSM) images were obtained to assess the fluorescence intensity of each group after transdermal delivery

What Are Copper Peptides for Collagen, and How Do They Work

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