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The vertical distance from the alar base on the normal side 4 to the height of the non-cleft peak of the bow 2 gives the distance that must be matched ultimately on the cleft side from its alar base 10 to its peak 8 (usually around 10 mm)

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The bRP fractions were run on for 110 min, including a linear 84-min gradient from 94.6% solvent A (0.1% formic acid) to 27% solvent B (99.9% acetonitrile, 0.1% formic acid), followed by a linear 9-min gradient from 27% solvent B to 54% solvent B