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Chapman I, Parker B, Doran S, Feinle-Bisset C, Wishart J, Strobel S, Wang Y, Burns C, Lush C, Weyer C, Horowitz M

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Sections were microwave treated for 8 min at 900 W and 30 min at 360 W in Tris-EDTA buffer, pH 9.0, were allowed to cool for 15 min, rinsed in Tris-buffered saline (TBS), pH 7.5, and blocked in 3% H 2 O 2 with 0.15 M NaN 3

Covariates Clinically relevant covariates, known to influence survival and cardiovascular or kidney outcomes, were carefully selected to ensure balanced comparisons between study groups