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2020, doi: 10.3390/molecules25030609

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Subsequently, PBMCs were transferred to a liquid nitrogen tank and stored in the vapor phase until further ex vivo reactivation and processing for CyTOF and flow cytometry

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A review study proposed another mechanisms underlying the potential interaction of the smoking and oestrogen metabolites such as decrease in oestrogen activity, SHBG levels, aromatase enzyme activity, C-20, 22 desmolase enzyme levels and increase in free testosterone levels, hyperandrogenism and the hepatic metabolism of steroid (Reference Ruan and Mueck41)