Multiplex Immunofluorescence, HALO analysis Multiplex immunofluorescence (mIF) was performed on 4 m FFPE sections of radical prostatectomies from men recruited to the Prostate Cancer Mechanisms of Progression and Treatment (ProMPT) study, as described in 32 and mIF methods in 41 , using Epitope Retrieval (ER) solutions, primary antibodies and their opal fluorophore pairings listed in Supplementary Table S2
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