To genotype the Gipr deletion, specific primers binding on the wild-type (WT) or deleted DNA region were designed and established ( Gipr -9-nt forward and reverse, and Gipr WT reverse primers) (ESM Table 3)
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Oxidative stress and antioxidant markers (vitamins and antioxidant enzymatic activity) were determined in plasma every month
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Because the testing involves sophisticated methods like mass spectrometry, it can take slightly longer than a basic metabolic panel or a complete blood count
