Alternative 20-day extended protocol Extended cycle approach: Duration: 20 consecutive days Dose: 5-10mg per day Total: 100-200mg per cycle Frequency: Every 6-12 months Goal: Deeper, more sustained effects Why 20 days: Allows more comprehensive cellular changes Telomere lengthening may be more pronounced Some users report stronger anti-aging benefits Less frequent cycling (only 1-2x per year) Higher total peptide investment 20-day protocol comparison: Who uses 20-day protocol: Users wanting longer cycles with less frequent dosing Those who respond well to extended peptide therapy Individuals combining with other anti-aging interventions People who prefer fewer total cycles Potential advantages: May produce more sustained benefits Fewer cycles = less injection burden Possibly stronger gene expression changes Some report better sleep normalization Potential disadvantages: Higher per-cycle cost More injection days consecutively Less opportunity to assess response before next cycle No evidence it's superior to 10-day protocol Learn about cycling different peptides at SeekPeptides
Nature 302 , 716718 (1983)
Started with 0.25 mg twice daily, subcutaneous injections near the shoulder
Specifically, as an endogenous ligand of the aromatic hydrocarbon receptor, trans-3-indoleacrylic acid upregulates the expression of aldehyde dehydrogenase 1 family member A3, leading to increased production of NADH by utilizing retinol as a substrate
(2018) Potential use of TIA-1, MFF, microRNA-200a-3p, and microRNA-27 as a novel marker for hepatocellular carcinoma, Biochem
Mechanism of Action Tyrosine Hydroxylase Pathway : NALT converts to L-tyrosine in the body, which then undergoes hydroxylation by tyrosine hydroxylase to form L-DOPA, the direct precursor to dopamine synthesis in the brain
