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How it works: AOD 9604 is a small piece of human growth hormone (amino acids 177-191), specifically the fat-burning fragment

(18(2)) doi:10.3390/nu18020271

This proposed mechanism has been directly validated in animal models where the surgically severed vagus nerve attenuated anxiety- and depression-like behaviors in mice after oral antibiotic administration, providing direct evidence for neuropsychiatric effects induced by antibiotics through the gutbrain axis (Joo et al., 2023)

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Mohammed Taouis, Yacir Benomar Molecular and Cellular Endocrinology.2021

Carbonyls were derivatised with 2,4-dinitrophenyl hydrazine (Sigma-Aldrich) and coated on an ELISA plate (Nunc-Immuno plate maxisorp, Nunc, Roskilde, Denmark)