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Sipeimine and rosavin prevent ferroptosis by reducing the levels of Fe 2+ , MDA, and inflammatory factors, offering therapeutic benefits in lung injury through phosphoinositide-3-kinase (PI3K)/protein kinase B (AKT)-mediated Nrf2 expression

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After three PBST washes, 50 L of SpyTag-tagged pHLA-I (0.5 M in blocking buffer) was added to each well and incubated for 1 h at RT, followed by three additional PBST washes

Walter RB, Milano F, Brasky TM, White E