At 48 hours, you're still within that window so the picture isn't a neat "peak then decline"
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It is conceivable that the GAP activity observed in our in vitro assays may be further stimulated in vivo by interactions of TBC1D9B with other proteins (e.g., TMEM55B), by membrane lipids, and/ or via posttranslational modifications that could alter the conformational state and, thereby, the activity of TBC1D9B
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for non-functionalized nanoparticles, highlighting the importance of lactoferrin conjugation