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However, reactive oxygen species, which are central players in the APAP-induced signaling cascade, have been shown to be involved in RIP3-induced necroptosis in the cardiomyocyte [135] and several studies have also implicated RIP1 in APAP-induced liver injury [57,73,74,136] further confirming that the mode of cell death after APAP is regulated necrosis

For HPLC and LCMS analysis, this fraction was further diluted with water (MilliQ) by a factor of 10 ( v / v )

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Untargeted DDA with subsequent database searching did not require any custom-made standards

doi: 10.1093/jn/124.3.378