Quantification of GSH using Ellmans assay Cells were harvested and lysed with 0.1% Triton-X and 0.6% sulfosalicylic acid in 0.1 M potassium phosphate buffer with 5 mM EDTA at pH 7.5 to afford protein free lysate
Immunohistochemistry (IHC) Fresh human specimens were fixed with 4% paraformaldehyde solution for 24 h, embedded in paraffin, and cut into sections (5 m), which were then immersed in xylene and ethanol for deparaffinization and rehydration, respectively
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This is a lyophilized powder that typically requires reconstitution with a suitable sterile diluent before use, and careful handling to reduce contamination
[DOI] [PMC free article] [PubMed] [Google Scholar] 62.Selek S., Savas H.A., Gergerlioglu H.S., Bulut M., Yilmaz H.R
The blood-brain barrier, when intact, filters most contaminants