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By exploring the changes in gut microbiota and enzyme activity in mice with diarrhea with kidney-yang deficiency syndrome, the aim is to elucidate the underlying mechanisms of diarrhea occurrence from the perspective of gut microbiota and enzyme activity

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Primary antibodies against GAPDH (2118-T, 1:10000 dilution) and PG (D16G10, 1:1000 dilution) were obtained from Cell Signaling Technology (Shanghai, China)