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Upon elution, the mixture is resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and analyzed by Coomassie staining, silver staining, or Western blot

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Grx-catalyzed (de)glutathionylation is an important event in signal transductions and serves as the primary protective mechanism against the irreversible oxidation of cysteine residues [115]

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