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Subsequently, in 2016, Sijbrandi et al

doi: 10.1186/1423-0127-19-32 55 StasiMAScioliMGArcuriGMatteraGGLombardoKMarcelliniMet al

The IgV domain of PILRa was expressed with an N-terminal His 14 -NEDD8 tag in BL21DE Rosetta2 cells using autoinduction in Terrific Broth medium overnight at 25 C

LaccID was generated through 11 rounds of directed evolution from an ancestral fungal laccase and catalyzes one-electron oxidation of diverse aromatic substrates using O 2 instead of toxic H 2 O 2

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