3.2.2 Hemopexin interactome in HL-60 cells The well controlled heme-HPX affinity chromatography of cell extracts containing biotinylated surface proteins from undifferentiated human HL-60 cells, together with an unbiased analysis using LC-MS/MS, revealed some additional members of the HL-60 HPX interactome, providing novel and unique information on the HPX system in immune cells (Supplementary Table S1)
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however, IFN- (p = 0.011) and IL-12p70 (p = 0.048) remained significantly elevated, consistent with a sustained Th1-polarising signal
GAD patients demonstrated broader SCFA depletion, with decreased levels of Faecalibacterium, Eubacterium rectale, Butyricicoccus , and Lachnospira , bacteria integral to maintaining mucosal homeostasis and modulating host-microbiota immune interactions
