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For TNP-specific antibody detection, plates were coated at 4 C overnight with NP (7) -BSA (Biosearch Technologies N-5050L) at 1 g/mL

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GSH is continuously produced by glutamate cysteine ligase (GCL) and glutathione synthetase (GSS), and GCL activity is the rate-limiting step in GSH synthesis (Thompson et al., 2009

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doi.org/10.1155/2016/9537632 Showell MG, Mackenzie-Proctor R, Jordan V, Hodgson R, Farquhar C