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55:503-514 [PubMed: 29484489] 213
Compared with the control group, the treated cells can enhance the phosphorylation of the Akt signal 24 hours before differentiation [80]
In advance, 100 L of homogenates (sample tubes) or 100 L of 50 mM TrisHCl buffer, pH = 7.4 (control tube), were introduced into the test tubes, followed by 1,500 L of Ellmans reagent
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Taken together, FOXO4-DRI is able to remove the senescent cells in PDL9 chondrocytes, but its utility in promoting cartilage formation from the in vitro expanded chondrocytes needs further investigation Given that FOXO4 expression may be a biomarker for postmenopausal osteoporisis, perhaps the founding researchers were onto something with their discovery
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